Fig 3.

In vitro phenotypic assays of the parental strains XL280p and JEC20a and the two congenic strains XL280α and XL280a. Yeast cells of each strain were grown in liquid YPD medium overnight, and serial dilutions (10-fold) of cells were spotted onto different media. Cells were grown on minimal YNB medium at 22°C for 3 days as a control for growth (first column from the left); cells were grown on DMEM (DME) at 37°C under 5% CO₂ to assay growth under host-relevant conditions (2nd column) and capsule production (images below); cells were grown on the l-DOPA medium at 22°C for 6 days (3rd column) to assay melanin production (brown pigment); cells were grown on YNB supplemented with 1 M NaCl or with 1 mM H₂O₂ to assay resistance to osmotic stress (4th column) or oxidative stress (last column). Self-filamentation of these strains on the L-DOPA medium is shown below.