Fig 7.

The mating type distribution during a-α coinfection in the intravenous infection model of cryptococcosis. (A) Animals were challenged with the mixture of a-α fungal cells (1 × 10⁶) intravenously. At the time of termination, brains, kidneys, and spleens from seven animals per group were dissected and homogenized. The mating type of randomly picked Cryptococcus cells recovered from each organ was determined. (B) Animals were challenged with 1 × 10⁶ fungal cells (α, a, or the a-α mixture) intravenously. At day postinfection 5 and also the time of termination, brains, kidneys, and spleens from five animals per group were harvested. Serial dilutions of the homogenized tissue were plated, and CFU were used to determine the organ fungal burden. The short, bold, horizontal lines indicate the median. No statistically significant difference was found among the groups in the brain (P = 0.370), the kidney (P = 0.410), or the spleen (P = 0.070).