Fig 8.

Znf2 controls filamentation and the formation of complex colony morphology in XL280 background. (A) The wild type, the znf2Δ mutant, and the ZNF2^oe strain with the inoculum were cultured on complete YPD medium, minimum YNB medium, and V8 juice medium at 22°C in the dark for 2 days. Photographs of the colony edge were shown to display filamentation (fluffy edge) or yeast cells (smooth edge). (B) The wild type, the znf2Δ mutant, and the ZNF2^oe strain with the inoculum were cultured on YPD and YNB media at 22°C in the dark for 4 days. The wrinkled colony indicates the complex colony morphology. (C) The nonfilamentous strain JEC20a was mated with the wild type, the znf2Δ mutant, and the ZNF2^oe α strains on V8 juice agar medium. The mating mixtures were cultured at 22°C in the dark for 9 days before the photographs were taken. The white fluffy phenotype reflects aerial hyphal production in the mating colony. The cultures of the wild type, the znf2Δ mutant, and the ZNF2^oe α strains without a mating partner are shown below for comparison.