Fig 6.

(A) Quantification of nitrites in BAL fluid from mice treated with 1 μg of B. pertussis LPS alone or coadministered with the NOS inhibitor aminoguanidine (AG). BAL fluid samples were collected for nitrite determination 2 h after treatment. The results show the average concentrations for five mice per group. P values were <0.001 in the Bonferroni test. (B) Bacterial colonization upon treatment with the NOS inhibitor. Mice were treated with 1 μg of B. pertussis LPS coadministered with the NOS inhibitor 2 h before challenge with B. pertussis (10⁷ CFU/40 μl). The lungs of the infected mice were collected for bacterial counts 2 h after the challenge. The results show the average counts for five mice per group. The results are representative of at least four independent experiments. *, P < 0.001 in the Bonferroni test. ND, not detected.