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. 2013 Jul;81(7):2405–2414. doi: 10.1128/IAI.00238-13

Table 1.

Bacterial strains and plasmid characteristics

Strain or plasmid Relevant characteristic(s)a Reference or source
E. coli
    JIR10750 Top10(pJIR3872), Emr Transformant
C. perfringens
    CN1020 Type D wild type (pJIR3118) Burroughs-Wellcome collection via R. G. Wilkinson (20)
    JIR4981 CN1020(pJIR3120) etx::catP, Cmr/Thr Transformant
    JIR12604 JIR4981(pJIR3120, pJIR3872), Cmr/Thr, Emr
Plasmids This study
    pJIR988 E. coli-C. perfringens shuttle vector, Emr 20
    pJIR3118 Wild-type 48-kb ε-toxin plasmid from CN1020 20
    pJIR3120 pJIR3118 etx::catP, Cmr/Thr This study
    pJIR3872 pJIR988 Ω 1.4-kb etx+ PCR product, Emr
a

Cmr/Thr, chloramphenicol and thiamphenicol resistance; Emr, erythromycin resistance.