Table 1.
Bacterial strains and plasmid characteristics
Strain or plasmid | Relevant characteristic(s)a | Reference or source |
---|---|---|
E. coli | ||
JIR10750 | Top10(pJIR3872), Emr | Transformant |
C. perfringens | ||
CN1020 | Type D wild type (pJIR3118) | Burroughs-Wellcome collection via R. G. Wilkinson (20) |
JIR4981 | CN1020(pJIR3120) etx::catP, Cmr/Thr | Transformant |
JIR12604 | JIR4981(pJIR3120, pJIR3872), Cmr/Thr, Emr | |
Plasmids | This study | |
pJIR988 | E. coli-C. perfringens shuttle vector, Emr | 20 |
pJIR3118 | Wild-type 48-kb ε-toxin plasmid from CN1020 | 20 |
pJIR3120 | pJIR3118 etx::catP, Cmr/Thr | This study |
pJIR3872 | pJIR988 Ω 1.4-kb etx+ PCR product, Emr |
Cmr/Thr, chloramphenicol and thiamphenicol resistance; Emr, erythromycin resistance.