Fig 3.

HIF-1-dependent gene expression in HepG2 HIF-1α^−/− and HepG2 cells. Four hours after infection with S. aureus (MOI, 20), total RNA was isolated from HepG2 HIF-1α^−/− and HepG2 cells and was transcribed into cDNA, and the relative expression of the LOX, VEGF, HK2, PFKFB3, TET1, PHD3, and HIF-1α genes was determined by real-time PCR analysis in triplicate (filled bars). Expression was calculated in relation to that in noninfected HepG2 cells (open bars). The β-2-microglobulin gene (B2M) was used for normalization. Asterisks indicate significant differences from values for control cells (P < 0.01); ns, not significant.