Fig 4.

Induction of LOX upon S. aureus infection. (A) LOX expression in NHEKs upon S. aureus infection. NHEKs were infected with S. aureus (MOI, 20) for 4 h; total RNA was isolated and was transcribed to cDNA; and the relative expression of the LOX gene was determined using real-time PCR analysis. Expression was calculated relative to that in uninfected control cells (triplicates). The β-2-microglobulin gene (B2M) was used for normalization. Asterisks indicate significant differences from control cells (P < 0.01). (B) LOX secretion from S. aureus-infected HepG2 cells (MOI, 0.01). Supernatants were taken 16 h after infection and were analyzed by LOX-ELISA (triplicates). Asterisks indicate significant differences from control cells (P < 0.01).