Fig 5.

Detection of LOX in murine kidney abscesses due to S. aureus. NMRI mice were intravenously infected with S. aureus 8325-4 (1.0 × 10⁷ bacteria; 200 μl) for 5 to 7 days. Uninfected control mice were treated i.v. with sterile PBS (200 μl). (A) Lox expression in kidneys. Total RNA was isolated from kidneys, transcribed to cDNA, and analyzed by real-time PCR. Lox expression in infected mice (n = 12) was calculated relative to that in the uninfected, PBS-treated control group (n = 13). The hypoxanthine phosphoribosyltransferase gene (Hprt) was used for normalization. The asterisk indicates a significant difference from the control group (P < 0.01). (B through D) Hematoxylin-eosin (H/E) staining (B) and LOX immunohistochemistry (C and D) of kidney abscesses due to S. aureus. Images represent the results of two independent experiments, each with three control mice and three infected mice. Kidneys were formalin fixed and paraffin embedded, and sections were stained with H&E and with a LOX-IHC stain. LOX-positive cells appear abundant at abscess borders. Arrows indicate abscesses. Bars, 100 μm.