Fig 7.

Influence of the LOX inhibitor BAPN on kidney abscess morphology. NMRI mice were infected i.v. with S. aureus (∼1 × 10⁷ bacteria; 200 μl) for 5 days and were treated i.p. daily with the LOX inhibitor BAPN (150 mg/kg; 200 μl) starting 1 day before infection. Control mice (also infected with S. aureus) were treated with sterile PBS (200 μl). Five days after infection, kidneys were excised, formalin fixed, and paraffin embedded, and sections were stained with H&E. (A) Sections were analyzed microscopically and were classified as follows: (i) defined (clear abscess formation), (ii) intermediate, and (iii) diffuse (massive infiltration of granulocytes in the surrounding tissue without a clear abscess border). Bar, 100 μm. (B) Percentages of different abscess phenotypes from two independent experiments. Abscesses (100 abscesses from the kidneys of 12 mice treated with PBS and 123 abscesses from the kidneys of 10 mice treated with BAPN) were quantified microscopically, and the percentage of each phenotype was determined microscopically (*, P < 0.05).