Table 1.
HIF-1-regulated genes in HepG2 cells 4 h after S. aureus infection
| No. | Protein | Genea | Differential inductionb | Biological functionc (reference) | Reference sequence | Known HIF-1 target (reference) |
|---|---|---|---|---|---|---|
| 1 | DNA damage-inducible transcript 4 | DDIT4 | 5.18 | Stress responsec | NM_019058 | Yes (50) |
| 2 | 6-Phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 | PFKFB3 | 3.97 | Energy metabolismc | NM_004566 | Yes (51) |
| 3 | 6-Phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 | PFKFB4 | 3.46 | Energy metabolismc | NM_004567 | Yes (51) |
| 4 | Pyruvate dehydrogenase kinase, isozyme 1 | PDK1 | 3.02 | Energy metabolismc | NM_002610 | Yes (52) |
| 5 | Fucosyltransferase 11 [α(1,3) fucosyltransferase] | FUT11 | 2.70 | Fucosylation (53) | NM_173540 | No |
| 6 | Protein phosphatase 1, regulatory (inhibitor) subunit 3C | PPP1R3C | 2.51 | Glycogen synthesisc | NM_005398 | Yes (54) |
| 7 | BCL2/adenovirus E1B 19-kDa interacting protein 3-like | similar to p40 | BNIP3L | LOC440258 | 2.38 | Autophagy (55) | NM_004331 | Yes (56) |
| 8 | Hexokinase 2 | HK2 | 2.31 | Glucose metabolismc | NM_000189 | Yes (52) |
| 9 | Egl nine homolog 3 (Caenorhabditis elegans) | EGLN3 | 2.05 | Prolyl hydroxylase 3, hypoxic regulationc | NM_022073 | Yes (57) |
| 10 | Lysyl oxidase | LOX | 2.03 | Modulator of extracellular matrix, progression and metastasis of breast cancer (21, 44, 58) | NM_002317 | Yes (21) |
| 11 | Tet oncogene 1 protein | TET1 | 1.98 | Potential epigenetic regulatorc | NM_030625 | No |
| 12 | Vascular endothelial growth factor A | VEGFA | 1.89 | Angiogenesis (42) | NM_001025366 | NM_003376 | NM_001025367 | NM_001025368 | NM_001025369 | NM_001025370 | NM_001033756 | Yes (59) |
| 13 | Ankyrin repeat and zinc finger domain containing 1 | ANKZF1 | 1.83 | Unknown | NM_018089 | NM_001042410 | Yes (60) |
| 14 | Jumonji domain containing 1A | JMJD1A | 1.71 | Demethylasec | NM_018433 | Yes (61) |
| 15 | Basic helix-loop-helix domain containing, class B, 2 | BHLHE40 | 1.71 | Differentiationc | NM_003670 | Yes (62) |
| 16 | Tubulin beta-1 chain | TUBB1 | 1.65 | Platelet assemblyc | NM_030773 | No |
| 17 | Hypoxia-inducible protein 2 | HIG2 | 1.69 | Cell growth, lipid droplet protein (63, 64) | NM_013332 | NM_001098786 | Yes (64) |
| 18 | Adipose differentiation-related protein | PLIN2/ADFP | 1.66 | Lipid droplet protein (65) | NM_001122 | Yes (62) |
| 19 | Stanniocalcin 2 | STC2 | 1.62 | Potential role for glucose homeostasis and cell proliferation (66, 67) | NM_003714 | Yes (67) |
| 20 | Ras-like without CAAX 1 | RIT1 | 1.59 | GTPasec | NM_006912 | Yes (68) |
| 21 | Zinc finger protein 292 | ZNF292 | 1.53 | Zinc finger protein, unknown function (69) | NM_015021 | Yes (62) |
| 22 | Solute carrier family 16, member 6 (monocarboxylic acid transporter 7) | SLC16A6 | −2.05 | Potential monocarboxylic acid transporterc | NM_004694 | Yes (70) |
| 23 | Cysteine-rich, angiogenic inducer, 61 | CYR61 | −1.65 | Vasculo- and angiogenesisc | NM_001554 | Yes (70) |
| 24 | Keratin 18 pseudogene 49 protein | KRT18P49 | −1.63 | Unknown function | XR_016590 | XR_018216 | XM_001714362 | No |
a
Three independent data sets were analyzed on Affymetrix Human Gene ST 1.0 arrays by using GeneSpring, version 9.3, as described in Materials and Methods.
b
Differential expression in HepG2 and HepG2 HIF-1α−/− cells after S. aureus infection.
c
According to OMIM.