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TLR4-mediated downstream signaling pathways after B. abortus infection. (A to D) Immunoblot analysis of total cell lysates was assessed with phosphospecific and pan-specific antibodies against JAK2, ERK1/2, p38α, JNK, PI3K, and AKT in RAW 264.7 cells pretreated with AG490 (75 μM) (A) or E. coli LPS (100 ng/ml) (B) and WT or TLR4−/− BMDMs pretreated with AG490 (C) or E. coli LPS (D) at the indicated times p.i. Images shown are representative of three independent experiments.
