Fig 1.
SDS-PAGE and Western blot analysis of purified recombinant G. duodenalis ADI. (A) SDS-PAGE and Coomassie staining of affinity-purified recombinant catalytically inactive ADIC424A (lane 1), recombinant enzymatically active ADI (lane 2), and molecular mass standards (lane 3). (B) Antigenic identification of 0.5 μg affinity-purified recombinant ADI (lane 4), 0.5 μg catalytically inactive ADIC424A (lane 5), and native ADI in 2.9 μg of G. duodenalis strain WB-C6 lysate (lane 6) by Western blotting with alpaca polyclonal antiserum raised against ADI. Preimmune serum, used as a control, did not react with any G. duodenalis protein (data not shown).