Fig 2.

4HA-SdhA is found on LCVs in infected hemocytes. Hemocytes were extracted from G. mellonella larvae infected with ΔsdhA or ΔsdhA plus p4HA-SdhA L. pneumophila 130b at 5 or 18 h p.i. and stained using anti-HA (red) and anti-Legionella (green) antibodies. 4′,6-Diaimidino-2-phenylindole (DAPI) was used to visualize DNA (blue). (A) At 5 h p.i., 4HA-SdhA localized to the LCV membrane and was concentrated at the poles of the bacteria in approximately 45% of infected cells. (B) At 18 h p.i., 4HA-SdhA could be seen surrounding the bacteria. Results are representative of three independent experiments. Bar, 5 μm.