Table 1.
CEACAM- and HSPG-binding profile of N. gonorrhoeae strain MS11 and strain VP1 Opa proteins
| Receptor | Bindinga |
|||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MS11 |
VP1 |
|||||||||||||||||||||
| Opa50 | Opa51 | Opa52 | Opa53 | Opa54 | Opa55 | Opa56 | Opa57 | Opa58 | Opa59 | Opa60 | Opa65 | Opa66b | Opa67 | Opa68 | Opa69 | Opa70 | Opa71 | Opa72 | Opa73 | Opa74 | Opa75 | |
| HSPG | +++ | − | − | − | − | − | − | − | − | − | − | − | − | − | +++ | ND | ND | ND | ND | ND | ND | ND |
| CEACAM1 | − | − | +++ | − | +++ | − | − | +++ | +++ | − | +++ | +++ | ND | − | − | − | +++ | − | +++ | + | +++ | +++ |
| CEA | − | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | − | ND | +++ | − | +++ | +++ | − | +++ | + | − | − |
| CEACAM6 | − | − | +++ | − | − | − | − | + | + | − | − | − | ND | + | − | − | + | − | − | − | − | − |
| CEACAM3 | − | − | +++ | − | − | − | − | +++ | +++ | − | − | − | ND | − | − | − | + | − | − | − | − | − |
a
CEACAM-binding properties of Opa proteins derived from N. gonorrhoeae strain MS11 or strain VP1 determined in this study using soluble, GFP-tagged amino-terminal CEACAM domains. +++, strong binding; +, weak binding; −, no binding; ND, not determined. Opa proteins showing strong CEACAM3 binding are indicated in bold. The HSPG-binding profile of Opa proteins was analyzed in the study by Kupsch et al. (30).
b
Opa66 was not analyzed in this study.