Fig 2.

V583, SV03 (V583 Δeep), SV14 (V583 ΔrsiV), WM02 (V583 ΔrsiV Δeep), and SV07 (V583 ΔsigV) strains were subjected to different stresses. (A) Strains were subjected to a temperature of 62°C. (B) Strains were subjected to a pH of 3.2 using 85% lactic acid. (C) Strains were subjected to 22% ethanol treatment. Strains subjected to lactic acid stress and ethanol stress were incubated at 37°C. Samples were drawn every hour for 2 h, were serially diluted in sterile PBS (pH 7.4), and plated onto THB agar. V583 Δeep and V583 ΔsigV were significantly attenuated compared to the growth of the wild-type (P < 0.001). Experiments were performed in triplicate, and error bars represent SEM.