Table 3.
Oligonucleotides used in this study
| Primer | Sequence (5′–3′)a |
|---|---|
| EepP1 | GAGAGGATCCGACATCAATGACACGTTGCC |
| EepP2 | CTCTTCTAGAGTTTTCATAGATGTCCTTCTTC |
| EepP3 | GAGATCTAGAACTTGGAACGATATTCAACGC |
| EepP4 | CTCTGCATGCCCACGAACCAAGACCATTAC |
| EepUp | TCTTTGGTGTACGGAGGACA |
| EepDown | GCATCTTCTTCTGTTGCTTC |
| Eep 5′ | GAGAGAATTCACTTTCATGGTAGCACATGTC |
| Eep 3′ | CTCTGGATCCCTGTTTCATTAAAACTCTCCTC |
| SigVP1 | GAGAGAATTCGAGCAGATTCGGAACTTTGAG |
| SigVP2 | CTCTGGATCCAACCATCGATTTCTGGAACCT |
| SigVP3 | GAGAGGATCCCTCCGAAGTCTATTGAATTAGT |
| SigVP4 | CTCTCTGCAGCAACTGACTTGGTTAGGTCAG |
| SigVUp | GTCACACATTGGCTTATAAGG |
| SigVDown | GCCACTTCTTCTTCGTTTCC |
| PgdAP1 | GAGAGAATTCGCTTGATTTGCTTGCAGTGC |
| PgdAP2 | CTCTGGATCCATGTCGCATACTTTCACTCCT |
| PgdAP3 | GAGAGGATCCTAGAGCAACTCGGAGCAC |
| PgdAP4 | CTCTCTGCAGGCCACCTTATGATCCAAGAG |
| PgdAUp | TCGCTTGGCTACTGTTGTGC |
| PgdADown | TTGCGAATACTCCTGAAGTAC |
| RsiVP1 | GAGAGGATCCCGGTATCTGTTGTTAATGGTG |
| RsiVP2 | CTTCTTCTAGACCGTTGGCAACGGTTGTTG |
| RsiVP3 | GAGATCTAGATGATTCCTGATCAAGTCATTG |
| RsiVP4 | CTCTCTGCAGCAATGACTTGGTCGTTGCTG |
| RsiVUp | CCGAGGAAGTCCTGCAAGG |
| RsiVDown | TCACTAATGGTAATGGTTGATC |
| RsiV5′ | GACTACAAAGACGATGACGACAAGCATATGGAAGATTTTGTAAAAAGTGTG |
| RsiV3′ | CTCTGCATGCGTCGCGTGTTTTTTACTGAGT |
| GFP5′ | GAGAGGATCCAAGGAGGAAAAACATATGAGTA |
| GFP3′ | CTCTATTAATTTTGTATAGTTCATCCATGCC |
| FLAGTAG | GAGAGGATCCAAGGAGGATTTATAGATGGATTATAAGGATCATGATTATAAGGATCATGATATCGACTACAAAGACGATGACGACAAG |
a
The underlined sequences denote restriction sites that were added to the template-directed sequences to facilitate cloning.