Table 3.
Primer | Sequence (5′–3′)a |
---|---|
EepP1 | GAGAGGATCCGACATCAATGACACGTTGCC |
EepP2 | CTCTTCTAGAGTTTTCATAGATGTCCTTCTTC |
EepP3 | GAGATCTAGAACTTGGAACGATATTCAACGC |
EepP4 | CTCTGCATGCCCACGAACCAAGACCATTAC |
EepUp | TCTTTGGTGTACGGAGGACA |
EepDown | GCATCTTCTTCTGTTGCTTC |
Eep 5′ | GAGAGAATTCACTTTCATGGTAGCACATGTC |
Eep 3′ | CTCTGGATCCCTGTTTCATTAAAACTCTCCTC |
SigVP1 | GAGAGAATTCGAGCAGATTCGGAACTTTGAG |
SigVP2 | CTCTGGATCCAACCATCGATTTCTGGAACCT |
SigVP3 | GAGAGGATCCCTCCGAAGTCTATTGAATTAGT |
SigVP4 | CTCTCTGCAGCAACTGACTTGGTTAGGTCAG |
SigVUp | GTCACACATTGGCTTATAAGG |
SigVDown | GCCACTTCTTCTTCGTTTCC |
PgdAP1 | GAGAGAATTCGCTTGATTTGCTTGCAGTGC |
PgdAP2 | CTCTGGATCCATGTCGCATACTTTCACTCCT |
PgdAP3 | GAGAGGATCCTAGAGCAACTCGGAGCAC |
PgdAP4 | CTCTCTGCAGGCCACCTTATGATCCAAGAG |
PgdAUp | TCGCTTGGCTACTGTTGTGC |
PgdADown | TTGCGAATACTCCTGAAGTAC |
RsiVP1 | GAGAGGATCCCGGTATCTGTTGTTAATGGTG |
RsiVP2 | CTTCTTCTAGACCGTTGGCAACGGTTGTTG |
RsiVP3 | GAGATCTAGATGATTCCTGATCAAGTCATTG |
RsiVP4 | CTCTCTGCAGCAATGACTTGGTCGTTGCTG |
RsiVUp | CCGAGGAAGTCCTGCAAGG |
RsiVDown | TCACTAATGGTAATGGTTGATC |
RsiV5′ | GACTACAAAGACGATGACGACAAGCATATGGAAGATTTTGTAAAAAGTGTG |
RsiV3′ | CTCTGCATGCGTCGCGTGTTTTTTACTGAGT |
GFP5′ | GAGAGGATCCAAGGAGGAAAAACATATGAGTA |
GFP3′ | CTCTATTAATTTTGTATAGTTCATCCATGCC |
FLAGTAG | GAGAGGATCCAAGGAGGATTTATAGATGGATTATAAGGATCATGATTATAAGGATCATGATATCGACTACAAAGACGATGACGACAAG |
The underlined sequences denote restriction sites that were added to the template-directed sequences to facilitate cloning.