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HPLC analysis of Ch1CoA dehydrogenase assay. The upper panel shows reactions catalyzed by Ch1CoA dehydrogenase. Oxidation of Ch1CoA (a) to Ch1,5CoA (b) in the presence of ferricenium (FcPF6) as an electron acceptor is shown. This product is further oxidized to benzoyl-CoA (c). HPLC diagrams of CoA ester analysis are shown after 0 min (A), 1 min (B), and 5 min (C). AU, absorption units.
