Fig 3.

Western blot immunodetection of GldK (A), GldL (B), GldM (C), and GldN (D) in whole-cell extracts of wild-type, mutant, and complemented strains of F. johnsoniae. The wild-type strain was CJ1827 (rpsL2), the gldK Himar mutant was CJ1372, and the strains with deletions in gldK, gldL, gldM, and gldNO were CJ2122, CJ2157, CJ2262, and CJ2090, respectively. pTB99, pTB81a, pTB94a, and pTB79 express GldK, GldL, GldM, and GldN, respectively, and thus complement the indicated mutants. Fifteen micrograms of protein was loaded into each lane.