Fig 5.
gldK, gldL, and gldM are each required for formation of spreading colonies. Colonies were grown for 36 h at 25°C on PY2 agar medium. Photomicrographs were taken with a Photometrics Cool-SNAPcf2 camera mounted on an Olympus IMT-2 phase-contrast microscope. The wild-type strain was CJ1827. CJ1827, CJ2122 (ΔgldK), CJ2157 (ΔgldL), and CJ2262 (ΔgldM) carried control vector pCP23 so that all strains were resistant to tetracycline and could be grown on identical media. pTB99, pTB81a, and pTB94a express GldK, GldL, and GldM, respectively. The bar indicates 0.5 mm and applies to all panels.