Fig 6.

Immunodetection of SprB and RemA in cells of wild-type and mutant F. johnsoniae strains. (A) Cell extracts (15 μg of protein) of wild-type (CJ1827), ΔsprB (CJ1922), ΔgldK (CJ2122), ΔgldL (CJ2157), and ΔgldM (CJ2262) strains were examined by Western blotting using antiserum against SprB. (B) Cell extracts (15 μg of protein) of wild-type (CJ2083), ΔremA (CJ1984), ΔgldK (CJ2140), ΔgldL (CJ2281), and ΔgldM (CJ2263) strains were examined by Western blotting using antiserum against RemA. All strains in panel B except the ΔremA strain (CJ1984) were derived from CJ2083 and thus expressed the Myc-tagged version of RemA.