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HPLC analysis of the degradation products formed during enzyme assay with nPHB granules incubated with 1 mM CoA. nPHB granules were accumulated in E. coli during simultaneous expression of phaP1 and phaZ1 and were isolated and subjected to degradation assay as described in Materials and Methods. The obtained supernatant was filtered after 30 min of incubation, and 20 μl was analyzed by HPLC. Peaks were identified by comparison with standards, as indicated.
