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Assay sensitivity was assessed by amplification of an Accrometrix CMVtc panel. Gel electrophoresis (1.5% TBE gel) of ROI 1 PCR, representative of all regions, using an Accrometrix CMVtc panel as the template. Lanes 1 to 5 represent panel components as follows: 1, negative control; 2, 240 IU/ml; 3, 2,400 IU/ml; 4, 24,000 IU/ml; 5, 240,000 IU/ml. Lane 6 is a CMV-positive control. Equivalent results were obtained for all UL97 and UL54 ROIs.
