Table 1.
Frequencies and densities of Treponema phylotypes in DD biopsy samples
| Phylotype | Pyrosequencing results |
FISH results |
||
|---|---|---|---|---|
| Frequency (%) (n = 36)a | Mean densityb | Frequency (%) (n = 37)c | Mean density scored | |
| T. phagedenis (PT6) | 100 | 0.19 | 95 | 2.54 |
| T. medium/T. vincentii (PT5) | 86 | 0.16 | 73 | 2.22 |
| T. denticola (PT7) | 86 | 0.13 | 63 | 1.96 |
| PT9 | 81 | 0.08 | 81 | 2.10 |
| Treponema clone 9T-42 | 67 | 0.02 | NDe | ND |
| PT4 | 61 | 0.09 | 62 | 1.70 |
| T. pedis (PT16) | 44 | 0.31 | 24 | 2.56 |
| PT18 | 42 | 0.21 | 59 | 2.55 |
| PT8 | 39 | 0.23 | 57 | 1.90 |
| Treponema clone 517 | 39 | 0.05 | 5 | 1.00 |
| T. refringens | 28 | 0.03 | 68 | 1.52 |
| PT12/PN20 | 25 | 0.21 | 3 | 2.00 |
| PT1 | 22 | 0.04 | 19 | 1.43 |
| PT15 | 22 | 0.07 | 5 | 1.50 |
| Treponema clone 356 | 17 | 0.02 | ND | ND |
| PT2 | 14 | 0.03 | 8 | 1.00 |
| PT13 | 11 | 0.04 | 11 | 1.50 |
| Treponema clone 198 | 11 | 0.03 | ND | ND |
| PT3 | 6 | 0.01 | 0 | 0 |
| Treponema clone 2.82 | 3 | 0.02 | ND | ND |
a
Values represent the proportions of positive biopsy samples. Findings for cows 1 to 4 were not included in the analysis.
b
Mean density values for positive biopsy samples.
c
Findings for cows 2, 7, and 8 were not included in the analysis.
d
Mean density scores (from 1 to 3) for positive biopsy samples.
e
ND, not determined.