Fig 1.

Recovery of 36 serotype 19F isolates that were cmPCR nontypeable from carriage and invasive sources. Six hundred twenty pneumococcal carriage isolates were obtained from a survey conducted in Goiânia city from December 2010 through February 2011 (each isolate from a separate child). Of these isolates, 13 were cmPCR nontypeable (cmPCR-NT) and serotype 19F. Three hundred ninety-two isolates from 200 children that were less than 5 years of age were collected in Salvador city during 2008 (multiple swabs from some children at 3-month intervals), 20 of which were cmPCR-NT and serotype 19F (18 of which were from a separate child). All 52 isolates recovered from CSF or blood represented independent infections occurring in Salvador city between 2008 and 2010. All 36 cmPCR-NT, serotype 19F isolates, and, in addition, the previously described 19F sequence variant 2584-08 (5), were PCR typeable using a new primer set derived from their wzy sequences (GenBank accession numbers KC690152 and FJ829071). NP, nasopharyngeal; CSF, cerebrospinal fluid.