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(A) Identification of E protein from transformed cells by SDS-PAGE. Lane 1, Sf9 expressing pFastBac-E; lane 2, Sf9 expressing pFastBac; lane 3, molecular mass marker. (B) Purified His-E protein analyzed by SDS-PAGE and detected by Western blotting with duck anti-tembusu virus sera. Lane 1, molecular mass marker; lane 2, purified His-E protein; lane 3, protein from pFastBac-transformed Sf9 cells.
