Table 1.
Summary of 11 years of bacterial identification in our laboratorya
| Identification technique (study period [day-mo-yr]) | Study period (no. of months) | Total no. of analyses | No. of clonal isolates | No. of isolates identified by 1st PID | No. of species identified by 1st PID | No. of bacterial species identified/year | No. of isolates confirmed by 2nd PID | No. of isolates identified by molecular identification | No. of isolates misidentified by 1st PID | % misidentified |
|---|---|---|---|---|---|---|---|---|---|---|
| CPI period (1-Jan-02 to 30-Aug-09) | 91 | 322,291 | 175,999 | 174,636 | 336 | 44 | 620 | 743 | 1,363 | 0.77 |
| MALDI-TOF MS period (1-Sep-09 to 30-Dec-12) | 40 | 177,888 | 110,843 | 110,263 | 382 | 112 | 50 | 530 | 580 | 0.52 |
| AutoFlex II (1-Sep-09 to 30-Nov-10) | 15 | 52,695 | 34,839 | 34,497 | 264 | 211 | 32 | 310 | 342 | 0.98 |
| MicroFlex (1-Dec-10 to 31-Dec-12) | 25 | 125,193 | 76,004 | 75,766 | 340 | 163 | 18 | 220 | 238 | 0.31 |
| Total | 131 | 500,179 | 286,842 | 284,899 | 459 | 42 | 670 | 1,273 | 1,951 | 0.68 |
a
We identified 459 bacterial species among 284,899 clinical isolates during nearly 11 years. We identified 112 species per year using MALDI-TOF MS compared with 44 identified using conventional phenotypic identification (CPI) (Gram staining, API, Vitek 2 system identification). PID, phenotypic identification.