Table 4.

Comparison of the pan-DENV, Altona, multiplex rRT-PCR, and heminested assays with a composite reference

Assay	Assay result	Composite reference results (n)
		Positive	Negative	Total
Pan-DENV	Positive	148	7	155
	Negative	3	42	45
	Total	151	49	200
Altonab	Positive	94	0	94
	Negative	36	21	57
	Total	130	21	151
Multiplex rRT-PCR	Positive	151	6	157
	Negative	0	43	43
	Total	151	49	200
Heminested	Positive	119	3	122
	Negative	32	46	78
	Total	151	49	200

^aA single sample failed RNase P amplification and was excluded from all comparisons.

^bForty-nine IC failures were excluded from the Altona analysis.