Table 2.
The DCFH oxidation (nMole mg−1 hr−1 ) of cell families B and H after addition of 100 µM H2O2 and 250 nM thimerosal. ASD B-cells from family B showed a significant difference (P = 0.01) of DCFH oxidation when exposed to thimerosal. None of the other cells lines in B or H showed any statistical difference. This indicates an inherent defect of antioxidant defense in the ASD population of cells.
| Cell ID | DCFH oxidation with 100 µM H2O2 | DCFH oxidation with 100 µM H2O2 and 250 nM Thimerosal | Difference |
|---|---|---|---|
| ASD-H | 49.85 ± 4.34 | 48.25 ± 2.02 | −1.61 |
| Twin-H | 49.32 ± 6.19 | 49.31 ± 2.42 | −0.01 |
| Sib-H | 48.35 ± 6.7 | 43.34 ± 2.35 | −5.01 |
| Cont-H | 47.15 ± 4.8 | 41.48 ± 5.46 | −5.67 |
|
| |||
| ASD-B | 83.5 ± 13.76 | 93.86 ± 13.76 | 10.396** |
| Twin-B | 61.32 ± 6.19 | 60.41 ± 10.69 | 2.963 |
| Sib-B | 62.55 ± 6.7 | 58.22 ± 10.81 | 3.816 |
| Cont-B | 46.93± 4.8 | 42.03 ± 17.6 | 4.990 |
**Indicates statistically significant difference.