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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Stem Cell Res. 2013 Apr 1;11(1):647–656. doi: 10.1016/j.scr.2013.02.009

Figure 1. Inducible Nkx2.1 expression in mESCs.

Figure 1

A. Schematic of the iNkx2.1 mESC line. B. Fluorescent in situ hybridization (FISH) of iNkx2.1 line demonstrating a single Lhx6∷GFP BAC insertion in chromosome 1 (with the native Lhx6 gene on chromosome 2). C. iNkx2.1 mESCs were cultured for 2 days, treated with Dox for 24 hours, and then fixed. Nearly all iNkx2.1 ESCs express Nkx2.1. Scale bar = 50 μm.