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. 2013 Jun 3;2(7):534–544. doi: 10.5966/sctm.2012-0134

Figure 1.

Figure 1.

SFM-1 increased and stabilized the TER and increased the expression of claudin-3 and claudin-19. (A): SFM-1 supports a TER that approximates that of hfRPE. At time zero, three cultures were transferred to SFM-1 (open symbols), while three cultures remained in KSR (closed symbols). Error bars indicate the SE and are sometimes smaller than the symbol. (B): Immunoblots demonstrate that steady-state levels of claudin-3 and claudin-19 increased in SFM-1. For comparison, preparations in which TER was unresponsive to SFM-1 are included. Abbreviations: Cdh1, epithelial-cadherin; Cdh2, neural-cadherin; Cldn, claudin; hESC, human embryonic stem cell; hfRPE, human fetal retinal pigment epithelium; KSR, knockout serum replacement medium; RPE, retinal pigment epithelium; SFM, serum-free medium; TER, transepithelial electrical resistance; unresp., unresponsive.