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IFN-αincreased STAT1 and SOCS1 mRNA levels, but decreased STAT3 mRNA in HepG2, MHCC97L, and HCCLM3 cells. The HCC and L02 cells were cultured with PBS, IFN-α, or both IFN-α and SG600-IL-24 for 48 hours. Then, RT-PCR assessed the mRNA levels of STAT1, SOCS1, and STAT3, along with β-actin, the internal control.
