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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Nature. 2011 May 11;474(7350):235–238. doi: 10.1038/nature09980

Figure 2. SecDF-dependent translocation completion.

Figure 2

a, Identification of a SecDF- and PMF-dependent translocation step. SecDF-deficient (secD1) IMVs were incubated with 35S-labeled proOmpA(L59) to generate translocation intermediates. The protein translocation was continued in the presence or absence of ATP, PMF and DTT. b, Schematic depiction of the translocation intermediate of proOmpA(L59). c, Completion of the proOmpA(L59) translocation using IMVs from the secD1 (Cs) mutant expressing E. coli SecDF derivatives.