Table 2.
Genes significantly regulated only in the atfer 1-3-4++ versus WT++ comparison. The 74 genes which are differentially expressed only in the atfer1-3-4++ versus WT++ comparison (see Fig. 1A) are classified into functional categories. The fold change of transcript content is given. Bold characters in fold changes indicate adjusted P-values <0.05; those not in bold indicate 0.05 < P < 0.1. The AGI codes, names, annotated functions, and/or domains, as well as metal binding of the corresponding proteins are listed. The column WT++ versus WT indicates regulation of genes in response to Fe excess in wild-type flowers (Fig. 1B). Genes that were also regulated in flowers of PIC1 overexpressors (Duy et al., 2011) are highlighted by a grey background; those that were re-analysed by qRT–PCR (Supplementary Table S1 at JXB online) are underlined.
| AGI code | atfer1-3-4++ versus WT++ | WT++ versus WT | Name, function/domains | Metal binding | |
|---|---|---|---|---|---|
| At1g23020 | Iron homeostasis | 1.4 | FRO3, ferric-chelate reductase | Fe | |
| At2g17360 | Protein metabolism+modification | –1.3 | RPS4A, 40S ribosomal protein S4 | ||
| At5g48650 | –1.5 | NTF2, nuclear transport factor 2 family protein/RNA recognition motif (RRM) | |||
| At5g47350 | –2.7 | Palmitoyl protein thioesterase family protein | |||
| At4g11890 | 2.6 | Protein kinase family protein | |||
| At5g58820 | –1.6 | Subtilisin-like serine endopeptidase family protein | |||
| At5g58840 | –1.8 | Subtilisin-like serine endopeptidase family protein | |||
| At4g12910 | –1.4 | scpl20, serine carboxy peptidase-like 20 | |||
| At1g13700 | Carbohydrate metabolism | 1.5 | Glucosamine/galacto samine-6-phosphate isomerase family protein | ||
| At1g19610 | Stress+redox | 2.0 | LCR78, PDF1.4, pathogenesis-related protein/plant defensin (PDF) family | ||
| At1g22900 | 1.7 | Disease resistance-responsive (dirigent-like protein)/molecular function unknown | |||
| At2g15120 | 4.9 | Pseudogene, disease- resistance family protein/fatty acid elongase-related | |||
| At5g17540 | –1.7 | HXXXD-type acyl-transferase family protein | |||
| At5g54220 | –1.4 | 1.7 | Defensin-like (DEFL) family protein | ||
| At1g21850 | –1.7 | sks8, copper ion-binding oxidoreductase | Cu | ||
| At1g30760 | –1.5 | FAD-binding domain-containing protein | |||
| At1g13110 | 1.3 | CYP71B7, cytochrome P450 family monooxygenase | Fe–haem | ||
| At2g12190 | 1.5 | Cytochrome P450 family monooxygenase | Fe–haem | ||
| At5g14130 | –1.9 | Peroxidase, putative | Fe–haem | ||
| At5g38900 | 1.8 | DSBA oxidoreductase family protein/thioredoxin superfamily | |||
| At2g28710 | DNA/RNA processes | 2.0 | Zinc finger (C2H2 type) family protein | Zn | |
| At4g32980 | 1.7 | ATH1, homeobox transcription factor | |||
| At5g26630 | –1.5 | AGL35, transcription factor/MADS-box protein | |||
| At1g09700 | –1.3 | HYL1, DRB1, double-stranded RNA binding/miRNA binding | |||
| At5g20850 | 1.6 | RAD51, DNA-dependent ATPase | |||
| At5g46590 | 1.4 | anac096, transcription factor/NAC domain-containing protein 96 | |||
| At5g38870 | –1.5 | Transposable element gene | |||
| At5g66280 | Cell wall modification | –1.6 | GMD1, GDP-mannose 4,6-dehydratase | ||
| At5g60490 | –1.6 | FLA12, fasciclin-like arabinogalactan protein/cell adhesion fasciclin domain | |||
| At1g26240 | 1.4 | Proline-rich extensin-like family protein | |||
| At2g45110 | –1.5 | EXPB4, expansin family protein | |||
| At5g39270 | –2.3 | EXPA22, expansin family protein | |||
| At1g11590 | –1.7 | Pectin methylesterase, putative | |||
| At4g03930 | –1.7 | 1.7 | Enzyme inhibitor/pectinesterase | ||
| At3g20470 | –1.7 | GRP5, gylcine-rich protein 5/structural constituent of cell wall | |||
| At3g28550 | 1.6 | –1.6 | Proline-rich extensin-like family protein | ||
| At4g22870 | Secondary metabolism | –1.7 | 1.7 | LDOX, ANS, leucoanthocyanidin dioxygenase/anthocyanidin synthase, 2-oxoglutarate and Fe(II)-dependent oxygenase | Fe |
| At1g61720 | –2.4 | BAN, oxidoreductase | |||
| At5g42800 | –1.8 | DFR, dihydrokaempferol 4-reductase | |||
| At1g19830 | Hormone metabolism | –1.5 | 1.6 | SAUR-like auxin-responsive protein, putative | |
| At2g46530 | –1.5 | ARF11, transcription factor auxin response | |||
| At1g63030 | –1.4 | ddf2, ethylene-responsive transcription factor/dwarf and delayed flowering/AP2-domain | |||
| At5g52020 | –1.8 | 1.6 | AP2-domain (apetala), ethylene-responsive transcription factor | ||
| At5g38970 | –1.6 | BR6OX1, brassinosteroid-6-oxidase1 / monooxygenase cytochrome P450 family | Fe–haem | ||
| At2g03760 | 1.3 | ST1, brassinosteroid sulfotransferase | |||
| At1g54450 | Signalling | –1.3 | Calcium-binding EF-hand family protein | Ca | |
| At2g16250 | –1.9 | Leucine-rich repeat transmembrane protein kinase, putative | |||
| At2g37330 | Transport | 1.4 | ALS3, ABC transporter-like permease protein, required for Al-tolerance | ||
| At1g53270 | –1.5 | ABCG10, ABC transporter family protein | |||
| At3g59030 | –1.9 | DTX41, TT12 (transparent testa), putative vacuolar flavonoid/H+ antiporter | |||
| At5g04770 | 1.3 | CAT6, cationic/basic amino acid transmembrane transporter | |||
| At1g30690 | –1.4 | SEC14, cytosolic factor family protein/phosphoglyceride transfer family | |||
| At1g08430 | 1.5 | ALMT1, aluminium-activated malate transporter | |||
| At4g17260 | Others | –1.4 | l-Lactate dehydrogenase, putative | ||
| At4g15480 | –1.5 | GT84A1, UDP-glycosyltransferase/sinapate 1-glucosyltransferase | |||
| At5g45700 | –1.6 | Haloacid dehalogenase-like hydrolase (HAD) superfamily/phosphatase | |||
| At2g12050 | 1.4 | –1.6 | Pseudogene, similar to aintegumenta-like protein | ||
| At1g64560 | –2.2 | Pseudogene of S-adenosylmethionine-dependent rRNA methyltransferase | |||
| At5g40830 | –1.2 | Methyltransferase | |||
| At2g24170 | Unknown function | –1.3 | 1.5 | EMP3, TMN10, putative TM9-type membrane protein of unknown function | |
| At1g68450 | 1.6 | VQ motif-containing protein | |||
| At2g22880 | –1.4 | VQ motif-containing protein | |||
| At3g05460 | –2.0 | Sporozoite surface protein-related | |||
| At5g12060 | –1.6 | Self-incompatibility protein-related | |||
| At2g05540 | 1.7 | Glycine-rich protein | |||
| At2g05580 | –1.7 | Molecular function unknown | |||
| At3g02670 | –2.0 | Proline-rich family protein | |||
| At1g15840 | –1.5 | Molecular function unknown | |||
| At1g65490 | 2.0 | Molecular function unknown | |||
| At3g02640 | –1.6 | Molecular function unknown | |||
| At3g14060 | 1.8 | Molecular function unknown | |||
| At3g21190 | –1.2 | Molecular function unknown | |||
| At4g09990 | –1.5 | Molecular function unknown | |||
| At5g36710 | –1.5 | Molecular function unknown |