Table 3.
Genes significantly regulated only in the atfer1-3-4++ versus WT comparison. The 43 genes which are differentially expressed only in the atfer1-3-4++ versus WT comparison (see Fig. 1A) are classified into functional categories. The fold change of transcript content is given. Bold characters in fold changes indicate adjusted P-values <0.05; those not in bold indicate 0.05 < P < 0.1. The AGI codes, names, annotated functions, and/or domains, as well as metal binding of the corresponding proteins are listed. The columns WT++ versus WT and atfer1-3-4 ++ versus atfer1-3-4 indicate regulation of genes in response to Fe excess in wild-type and mutant flowers, respectively (Fig. 1B). Genes that were also regulated in flowers of PIC1 overexpressors (Duy et al., 2011) are highlighted by a grey background; those that were re-analysed by qRT–PCR (Supplementary Table S1 at JXB online) are underlined.
| AGI code | atfer1-3-4 ++ versus WT | WT++ versus WT | atfer1-3-4 ++ versus atfer1-3-4 | Name, function/domains | Metal binding | |
|---|---|---|---|---|---|---|
| At3g03910 | Amino acid metabolism | –2.1 | –2.0 | GDH3, glutamate dehydrogenase 3 | ||
| AtCg01040 | Protein metabolism+ modification | 3.8 | YCF5, protein complex assembly/cytochrome c complex assembly | |||
| At2g47180 | Carbohydrate metabolism | –1.6 | –1.6 | GolS1, galactinol synthase1 raffinose family/glycosyl group transferase | ||
| AtCg00500 | Lipid metabolism | 7.9 | ACCD, carboxytransferase beta subunit of acetyl-CoA carboxylase (ACCase) complex in plastids | |||
| At5g33370 | 1.4 | GDSL-motif lipase/hydrolase family protein | ||||
| At1g09080 | Stress+redox | –2.2 | –1.9 | –2.6 | BIP3, ATP binding/heat shock protein 70 site/protein folding, response to heat, pollen tube growth | |
| At4g36040 | –1.3 | –1.5 | –1.5 | DNAJ heat shock N-terminal domain-containing protein | ||
| At2g47880 | –2.0 | Glutaredoxin family protein | ||||
| At5g64110 | 1.8 | Peroxidase, putative | ||||
| At2g14560 | –3.1 | LURP1, up-regulated in response to Hyaloperonospora parasitica | ||||
| At3g27060 | DNA/RNA processes | 1.6 | TSO2, oxidoreductase/ribonucleoside- diphosphate reductase | Fe | ||
| At3g27220 | –1.5 | MYB domain transcription factor/kelch repeat- containing protein | ||||
| At1g71030 | –1.5 | –1.8 | MYBL2, MYB-related transcription factor | |||
| At5g58900 | –1.5 | –1.5 | MYB-related transcription factor | |||
| At5g24470 | –2.0 | APRR5, transcription factor/two-component response regulator | ||||
| At1g78600 | –1.6 | LZF1, transcription factor/constans-like zinc finger family | Zn | |||
| At5g35970 | –1.6 | DNA-binding protein, putative | ||||
| At3g14530 | Secondary metabolism | 1.4 | 1.6 | Geranylgeranyl pyrophosphate synthase/isoprenoids, non-mevalonate pathway | ||
| At2g21220 | Hormone metabolism | 1.4 | 1.6 | SAUR-like auxin-responsive protein family/ calmodulin binding | ||
| At5g20820 | –1.8 | SAUR-like auxin-responsive protein family | ||||
| At3g23150 | –1.7 | ETR2, ethylene binding/histidine kinase receptor | ||||
| At4g17490 | –1.9 | –2.1 | ERF6, ethylene-responsive transcription factor | |||
| At5g61600 | –1.7 | ERF104, ethylene- responsive element-binding family/transcription factor | ||||
| At1g13260 | –2.0 | RAV1, AP2/B3-domain (apetala), ethylene-responsive transcription factor | ||||
| At5g39020 | Signalling | –1.6 | –1.4 | –1.4 | RLK1, receptor kinase family protein | |
| At1g73805 | –2.0 | Calmodulin binding | Ca | |||
| At2g03530 | Transport | –1.3 | –1.4 | UPS2, ureide permease/uracil transport | ||
| At1g27940 | 2.2 | ABCB13/MDR15/PGP13, full-type ABC transporter protein | ||||
| At3g43790 | –1.5 | ZIFL2, Zn-induced major facilitator protein/sugar: hydrogen symporter | ||||
| At2g04050 | 2.2 | DTX3, MATE efflux family protein | ||||
| At4g35770 | Others | –1.6 | –1.8 | SEN1, senescence- associated protein | ||
| At2g27080 | Unknown function | 1.7 | 2.0 | Late embryogenesis abundant (LEA) hydroxyproline-rich glycoprotein family | ||
| At2g30600 | –1.5 | –1.6 | BTB/POZ domain- containing protein | |||
| At1g13340 | 2.1 | Molecular function unknown | ||||
| At1g20070 | –1.6 | –1.4 | –1.5 | Molecular function unknown | ||
| At1g76240 | –2.2 | –1.5 | –1.6 | Molecular function unknown | ||
| At2g20670 | –1.5 | –1.5 | Molecular function unknown | |||
| At3g01860 | –1.5 | Molecular function unknown | ||||
| At3g22240 | –1.5 | Molecular function unknown | ||||
| At4g35720 | 1.6 | Molecular function unknown | ||||
| At5g05300 | –1.3 | –1.5 | Molecular function unknown | |||
| At5g57760 | –1.9 | Molecular function unknown | ||||
| At5g59080 | –1.8 | Molecular function unknown |