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Immunocytochemistry of glutelin in protein bodies. (A) Glutelin in PB-II in the wild type. (B, C) Glutelin localized on the normal PB-II and dPB in OsSar1abc RNAi lines. The black arrow indicates the dPB surrounded by rough ER membrane with ribosomes. (D) The white arrowhead indicates that glutelin did not accumulate in the blebbing structures of dPBs. Gold particles (10nm) indicate the reaction of glutelin antibody. Scale bars=0.5 μm in (A) and 200nm in (B–D).
