Table 1.
Histological observations during the compatible interaction between the stripe rust fungus and TaLls1-knockdown wheat plants. Significance was measured according to a paired sample t-test method (different lower-case letters indicate a significant difference: b*, P <0.01; b, P <0.05).
| Treatmenta | Hyphal lengthb | Infected areac | H2O2 d | Ratio of cell death (%)e | ||
|---|---|---|---|---|---|---|
| 24h p.i. | 48h p.i. | 120h p.i. | 120h p.i. | 24h p.i. | 120h p.i. | |
| BSMV: 00 | 1.54a | 2.68a | 11.7a | 5.24a | 3.60a | 1.95±0.76 |
| BSMV:TaLls1-as | 1.72a | 2.62a | 11.9a | 5.76a | 7.59b* | 7.89±1.31 |
| BSMV:TaLls1-as1 | 1.50a | 2.62a | 13.4a | 7.07a | 5.32b | 7.02±0.62 |
a Wheat leaves pre-infected with BSMV: 00 or the recombinant BSMV: TaLls1-as and BSMV: TaLls1-as1 followed by inoculation with Pst CYR31 in the compatible interaction.
b The average distance from the junction of the substomatal vesicle and the hyphal tip calculated from at least 50 infection sites (×10 μm).
c The average infected area of expanding hyphae plus the host cells calculated from at least 50 infection sites (×1000 μm2).
d The amount of H2O2 production was identified by DAB staining and measured by calculating the area of H2O2 produced in at least 50 infection sites (×100 μm2).
e Ratio of cell death at the infection sites counted from at least 50 infection sites.