Table 3.
Experiment | Barrel 1a | Barrel 2a | Direct ratiob | P valuec |
---|---|---|---|---|
A | TaLls1+GUS | EV+GUS | 0.42±0.08 | <0.01 |
B | TaLls11–178+GUS | EV+GUS | 0.35±0.07 | <0.01 |
C | TaLls1212–397+GUS | EV+GUS | 0.98±0.05 | >0.05 |
a Barrels 1 and 2 were physically identical and the masses of DNA in each barrel were identical. All of the replicates were conducted on the petiole-proximal half of the leaves and then the petiole-distal half. EV, empty vector.
b Ratios of blue spots that were counted in each barrel. Geometric averages and standard error were calculated from log ratios obtained from 16 pairs of shots.
c The P value for the direct comparison was calculated from the log ratios using a Wilcoxon signed-ranks test. A significant P value indicates that cell death was induced by TaLls1.