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. 2013 Jun 20;13:300. doi: 10.1186/1471-2407-13-300

Figure 2.

Figure 2

Immunoblot and immunohistochemical analysis of GnRH-I receptor (GnRH-IR) protein expression in endometrial cancer. (A) GnRH-I receptor levels in Ishikawa and ECC-1 cells were monitored by immunoblot assays. (B) GnRH-I receptor was stained brown in the second of three columns depicting human endometrial cancer tissue sections. Sections were counterstained with hematoxylin to show the nuclei in column 1 of three columns depicting human endometrial cancer tissue sections. Sections were stained without the GnRH-I receptor antibody as a negative control in the third of three columns depicting human endometrial cancer tissue sections. Micrographs were taken with a 40Χ objective lens. Scale bars represent 20 μm.