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. 2013 Jun 21;6:89. doi: 10.1186/1754-6834-6-89

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Copyright © 2013 Demeke et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Evaluation of the stability of D-xylose fermentation capacity in strain GS1.11-26. (a) Comparison of the number of colonies growing in YPD and YPX after growth for about 50 generations in non-selective conditions with only glucose as carbon source. Error bars represent standard deviation from three independent experiments. (b) Fermentation performance in YP + 40 g/L D-xylose as estimated from weight loss due to CO₂ release. Colonies 1 to 10 represent randomly selected single-cell clones isolated after growth of GS1.11-26 for 50 generations in YPD medium. The parent strain HDY.GUF5 is shown for comparison.