Figure 3.
D-xylose fermentation by superior single-cell isolates from different serial batch fermentations in the evolutionary engineering procedure. (a) CO2 production as measured by weight loss in semi-anaerobic batch fermentations in YP + 40 g/L xylose at 35°C. Selected single cell isolates from each of the 4th (GS1.4), 7th (GS1.7) and 11th (GS1.11) culture were used. The parent strain, HDY.GUF5, was used for comparison. (b) Final ethanol titer reached in the fermentations of (a). Each experiment was performed in duplicate, and error bars represent standard deviation from the average of duplicate values.