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The sequence, Tm and amplicon size of the designed primers used in PCR
| Primers | Target genes | Nucleotide sequence (5′ to 3′) | Tm (°C) | Amplicon (nt) |
|---|---|---|---|---|
| gD1F |
HSV-1 US6 |
AGACGTCCGGAAACAACCCTACAA |
64.6 |
752 |
| gD1R |
ACACAATTCCGCAAATGACCAGGG |
64.6 |
||
| GFPF |
HSV-1 GFP |
TGACCCTGAAGTTCATCTGCACCA |
64.6 |
717 |
| GFPR |
AACTCCAGCAGGACCATGTGAT |
62.7 |
||
| VP1F |
HSV-1 UL46 | ACCAAGCCTTGATGCTCAACTCCA |
64.6 |
957 |
| VP1R | ACAACACGGTTCCCGAGAGTTTGA | 64.6 |
