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. 2013 Jul 1;8(7):e69210. doi: 10.1371/journal.pone.0069210

Figure 8. Localization of MMPs and TIMPs in alveolar septa in the fetal rabbit model for CDH undergoing TO.

Figure 8

Representative images of immunohistochemistry for MMP2 (A–E), MMP14 (F–J), TIMP1 (K–O), and TIMP2 (P–T) in non-operated (A, F, Q, P), SHAM (B, G, L, K), DH (C, H, M, R), DH+ TO (D, I, N, S), and TO lungs (E, J, O, T). MMP2, MMP14, and TIMP2 were expressed in alveolar epithelial cells in non-operated, SHAM, DH+ TO, and TO lungs (high-magnification inserts). MMP2, MMP14, and TIMP2 were also shown in mesenchymal cells in the thickened septa of DH lungs (diffuse staining in-high magnification inserts). TIMP1 was localized in endothelial cells of capillaries in all study groups (solid arrowheads), and in some alveolar epithelial cells (open arrowheads) in non-operated, SHAM, and DH lungs (K, L, M). DH+ TO (N) and TO lungs (O) showed a much more diffuse immune reaction for TIMP1 in alveolar epithelial cells (open arrowheads) as compared to non-operated and SHAM lungs. Scale bar = 50 µm in A through T.