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. 2013 Jul 1;8(7):e67793. doi: 10.1371/journal.pone.0067793

Figure 1. Effects of HS-Csn8KO on deneddylation and CRLs activities in mouse liver at 4 weeks of age.

Figure 1

Liver tissue samples were collected from Csn8flox/flox::CreTG (referred to as HS-Csn8KO) and Csn8flox/flox::Cre NTG (referred to as CTL) littermate mice. (A) Representative images of western blot analyses for the indicated CRLs and their substrates. (B, C) Western blot analysis for hepatic total Nedd8 conjugates. Representative images (B) and pooled quantitative data (C) are presented. **p<0.01 vs. CTL. (D) Representative images of western blot analysis for hepatic total ubiquitin conjugates. Total ubiquitinated proteins were increased by >50% in HS-Csn8KO livers compared with the CTL (p<0.01). n=4 mice/group, student’s t-test.