Figure 5. Northern blot analyses of representative miRNAs in Aedes albopictus.

Nine representative miRNAs, miR-210 (A), miR-998 (B), miR-2941 (C), miR-133 (D), miR-2943 (E), miR-2946 (F), miR-1890 (G), miR-1891 (H) and miR-184 (I) were subjected to northern blot analyses. The top panels are the northern results and the bottom panels are RNA gels for verification of small ribosomal and tRNA integrity and equal loading of total RNA. ssDNA size markers (19 and 23 nts, not shown) also were visualized on the RNA gel for size estimation. Fifteen micrograms of total RNA were loaded for each sample. Abbreviations: E, embryos; L1–2, mixed 1^st and 2^nd instar larvae; L3–4, mixed 3^rd and 4^th instar larvae; P, pupae; M, adult males and F, adult females.