Table 3.
List of miRNAs over-expressed in prom1-exo compared with parental FEMX-I cells
| miRNA | Ct FEMX-Cells | Ct FEMX-Exosomes | DCt | Fold-charge |
|---|---|---|---|---|
| hsa-miR-216b |
>40 |
15.81 |
24.2 |
19138839.3 |
| hsa-miR-889 |
37.33 |
15.86 |
21.47 |
2910427.1 |
| hsa-miR-4307 |
>40 |
22.51 |
17.5 |
184083.4 |
| hsa-miR-4272 |
>40 |
22.99 |
17 |
131983.7 |
| hsa-miR-203 |
>40cyclcs |
23.82 |
16.2 |
74244.7 |
| hsa-miR-4289 |
23.34 |
8.67 |
14.66 |
25944.3 |
| hsa-miR-3149 |
22,7 |
8.77 |
13.94 |
15746.0 |
| hsa-miR-203 |
26.41 |
13.69 |
12.72 |
6769.4 |
| hsa-miR-3145 |
21.19 |
10.53 |
10.66 |
1622.1 |
| hsa-miR-1911 |
>40 |
29.72 |
10.3 |
1243.3 |
| hsa-miR-513a-3p |
>40 |
29.84 |
10.2 |
1144.1 |
| hsa-miR-3916 |
>40 |
30.52 |
9.48 |
714.1 |
| hsa-miR-886-3p |
>40 |
32.31 |
7.69 |
206.5 |
| hsa-miR-1182 |
22.77 |
15.91 |
6.86 |
115.1 |
| hsa-miR-3613-5p |
>40 |
33.69 |
6.31 |
79.3 |
| hsa-let-7i |
22.95 |
17.21 |
5.73 |
53.2 |
| hsa-miR-3132 |
16.50 |
11.49 |
5.01 |
32.2 |
| hsa-miR-3914 |
24.75 |
20.39 |
4.36 |
20.5 |
| hsa-miR-3618 |
28.56 |
24.35 |
4.21 |
18.5 |
| hsa-miR-1307 |
21.87 |
17.96 |
3.91 |
15.0 |
| hsa-miR-3614-3p |
21.90 |
19.15 |
2.75 |
6.7 |
| hsa-miR-519c-3p |
22.59 |
20.22 |
2.3k |
5.2 |
| hsa-miR-3160 |
17,61 |
15.28 |
2.33 |
5.0 |
| hsa-miR-3153 |
11.48 |
9.53 |
1.96 |
3.9 |
| hsa-miR-4278 |
18.94 |
16.99 |
1.95 |
3.9 |
| hsa-miR-3646 |
I.58 |
15.80 |
1.79 |
3.5 |
| hsa-miR-3926 |
17.47 |
15.72 |
1.75 |
3.4 |
| hsa-miR-515-5p |
28.37 |
26.69 |
1.68 |
3.2 |
| hsa-miR-3169 |
14.33 |
12.67 |
1.66 |
1.2 |
| hsa-miR-10a |
31.87 |
30.21 |
1.66 |
3.2 |
| hsa-miR-140-5p |
26.92 |
25.37 |
1.55 |
2.9 |
| hsa-miR-3148 |
18.74 |
17.56 |
1.18 |
2.3 |
| hsa-miR-4271 |
17.56 |
16.48 |
1.08 |
2.1 |
| hsa-miR-627 |
23.07 |
22.00 |
1.07 |
2.1 |
| hsa-miR-548d-3p |
29.69 |
28.66 |
1.03 |
2.0 |
| hsa-miR-3613-3p |
22.09 |
21.19 |
0.90 |
1.9 |
| hsa-miR-481 |
26.49 |
25.64 |
0.85 |
1.8 |
| hsa-miR-571 |
20.81 |
19.97 |
0.84 |
1.8 |
| hsa-miR-4274 |
19.93 |
19.15 |
0.79 |
1.7 |
| hsa-miR-4277 |
21.41 |
20.79 |
0.62 |
1.5 |
| hsa-miR-3686 |
15.41 |
14.81 |
0.61 |
1.5 |
| hsa-miR-3074 |
21.65 |
21.10 |
0.54 |
1.5 |
| hsa-miR-95 |
24.90 |
24.45 |
0.46 |
1.4 |
| hsa-miR-590-3p |
26.81 |
26.49 |
0.32 |
1.2 |
| hsa-miR-525-5p |
23.20 |
22.90 |
0.30 |
1.2 |
| hsa-miR-548g |
26.97 |
26.69 |
0.28 |
1.2 |
| hsa-miR-365 |
25.46 |
25.18 |
0.28 |
1.2 |
| hsa-miR-525-3p |
23.23 |
22.94 |
0.28 |
1.2 |
| hsa-miR-320d | 21.97 | 21.93 | 0.04 | 1.0 |
Total RNA from FEMX-I cells and exosomes was prepared with Qiazol extraction followed by poly-A tailing reactions and miRNA cDNA synthesis. 250 ng of cell’s total RNA and of exosomes’ RNA were used in cDNA synthesis. cDNA synthesis were carried out simultaneously and equal volume of cDNA synthesis reaction product was used in the subsequent real-time qPCR reactions. 1058 miRNAs were investigated. The DeltaCt (DCt) values for each miRNA-specific prom1-exo cDNA greater than 0.01 were listed.