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. 2013 Jul 1;8(7):e68045. doi: 10.1371/journal.pone.0068045

Figure 3. Functional effect of the “private” missense variations of UNC13D.

Figure 3

HMC-1 cells were transiently transfected with wild-type (WT) UNC13D and mutated forms carrying the (p.Cys112Ser, p.Val781Ile, p.Ile848Leu, p.Ala995Pro, p. Ile848Leu/p.Ala995Pro, and p.Pro271Ser) variations (C = untransfected cells). Twenty-four hours after transfection, cells were teated (or not) for 10 min with fMLP, and expression of CD63 was evaluated by flow cytometry. [A] Cytofluorimetric histograms of CD63 expression in fMLP-stimulated (black) and unstimulated (white) cells transfected with each construct; one experimental representative of six is shown. [B] Mean and SE of the fMLP-induced expression of CD63 from six experiments; results are relative to the CD63 expression displayed by unstimulated cells (set at 100%) in each experiment; the asterisk marks the statistically significant difference versus cells transfected with the WT form; *p<0.05; **p<0.01 vs MUNCwt transfected cells.