Figure 2. Casp-2S inhibits cisplatin-induced apoptosis.

Casp-2S was either over-expressed in A2780 and 2008 cells (A), or knocked down in CDDP cells and 2008C13 cells (B) for 48 h. Cells were treated with cisplatin for another 48 h, the cleaved PARP and cleaved casp-3 were detected in these cells by using Western blotting with anti-cleaved PARP and anti-cleaved casp-3 antibodies. Meanwhile, the expression of casp-2L and casp-2S was also detected. Tubulin was blotted to serve as a loading control.