Figure 4. Casp-2S interacts with cytoskeleton proteins.

A, Casp-2S was purified from HeLa cells stably transfected with the FLAG-tagged casp-2S using an anti-FLAG affinity gel. Purified proteins were separated by SDS-PAGE and stained with Coomassie blue. The differentially expressed bands in the HeLa-casp-2S cells were identified by mass-spectrometry. B, FLAG-tagged casp-2S was immunoprecipitated from the HeLa-Casp-2S cells and Fodrin was detected using Western blotting with the anti-Fodrin antibody. C, D, Fodrin was immunoprecipitated from the HeLa-Casp-2S cells (C) or CDDP cells (D) with an anti-Fodrin antibody, and the existence of casp-2S was detected with anti-casp-2S antibody.