Table 4.
Summary for primer sequences and product sizes
| Primer | Sequence (5’-3’) | Product size (bp) |
|---|---|---|
| Primers for RT-PCR | ||
|
GAPDH |
F:5'-CCACCCATGGCAAATTCCATGGCA-3' |
550 |
| R:5'-TCTAGACGGCAGGTCAGGTCCACC-3' | ||
|
BCAT1 |
F: 5'-CCAAAGCCCTGCTCTTTGTA-3' |
305 |
| R: 5'-TGGAGGAGTTGCCAGTTCTT-3' | ||
|
c-Myc |
F: 5'-CCTACCCTCTCAACGACAGC-3' |
179 |
| R: 5'-TTCCTCCTCAGAGTCGCTGC-3' | ||
|
KRAS |
F: 5'-GCAAAGACAAGACAGGGTG-3' |
264 |
| R: 5'-GGTAAAAGCTAACAGTCTGC-3' | ||
|
MCAM |
F: 5'- CTCCGCGTCTACAAAGCTCC-3' |
213 |
| R: 5'- ACCACTCGACTCCACAGTCT-3' | ||
| Primers for microsatellite loci of BCAT1 | ||
| D12S1435 |
F-CTTGTGCAACCCTCCCAC |
198 |
| R-ATATGTGCTGTGAATACATCCACC | ||
| D12S1617 |
F-AGCCTGAGGGGCCACAT |
259 |
| R-TGGGCAACTTGGATAAGAAACA | ||
| RH44650 | F-AAGAATGTGTCTATTGCCAGCA |
146 |
| R-CTCATGCCTCTGAAGGTTTTG | ||