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. 2013 Jun 22;10:205. doi: 10.1186/1743-422X-10-205

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Copyright © 2013 Morais et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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In vitro analyses of the YFV NS5(ID)-eIF3L interaction. (A) pGEX 5X1-ID plasmid, the GST-fused ID (33 kDa) expressed in E. coli BL21 (DE3). (B) Control GST (26 kDa) expressed in E. coli BL21 (DE3). (C) HeLa cells were transfected with a plasmid expressing Flag-tagged eIF3L (67 kDa) (column 1). This protein was incubated with resin-bound GST or GST-ID. After the samples were washed, the resin-bound proteins were resolved by SDS-PAGE and then analyzed by a western blot assay using an anti-Flag antibody. The interaction of Flag-eIF3L with GST-ID (column 3) was confirmed, without a nonspecific interaction with GST (column 2).